TMEM176B - TMEM176B

TMEM176B
Identifikatorlar
TaxalluslarTMEM176B, LR8, MS4B2, transmembran oqsili 176B
Tashqi identifikatorlarOMIM: 610385 MGI: 1916348 HomoloGene: 8521 Generkartalar: TMEM176B
Gen joylashuvi (odam)
Xromosoma 7 (odam)
Chr.Xromosoma 7 (odam)[1]
Xromosoma 7 (odam)
TMEM176B uchun genomik joylashuv
TMEM176B uchun genomik joylashuv
Band7q36.1Boshlang150,791,285 bp[1]
Oxiri150,801,360 bp[1]
Ortologlar
TurlarInsonSichqoncha
Entrez

28959

65963

Ansambl

ENSG00000106565

ENSMUSG00000029810

UniProt

Q3YBM2

Q9R1Q6

RefSeq (mRNA)
RefSeq (oqsil)
Joylashuv (UCSC)Chr 7: 150.79 - 150.8 MbChr 6: 48.83 - 48.84 Mb
PubMed qidirmoq[3][4]
Vikidata
Insonni ko'rish / tahrirlashSichqonchani ko'rish / tahrirlash

Transmembran oqsili 176B, yoki TMEM176B odamlarda TMEM176B geni tomonidan kodlangan transmembran oqsili. Dendritik hujayralarning pishib etish jarayonida muhim rol o'ynaydi deb o'ylashadi.[5]

Gen

Manzil

TMEM176B, shuningdek, LR8 va MS4B2 sifatida tanilgan.[6] Gen minus oxirida joylashgan 7-xromosoma, 7q36.1 pozitsiyasida uzun qo'lda.[7] Genning boshlang'ich pozitsiyasi 150,791,287 da va 150,801,360 ga to'g'ri keladi. Uning tarkibida 10 074 ta juftlik mavjud va jami 11 ta ekzons mavjud.[8]

Gen

TMEM176A va LOC105375566 TMEM176B qo'shnisi.[9][10]

Ifoda

Gen eng ko'p ifoda etilgan jigar bilan buyrak eng ko'p ifoda etilgan ikkinchi to'qima bo'lish.[6]

Ushbu rasm tanadagi turli to'qimalarda TMEM176B ekspression darajasini ko'rsatadi. [1]

Transkript variantlari

Ushbu genning izoform A va S variantlari bilan 3 ta izoformasi (A, B, C) mavjud. Isoform A 1-variantida 270 aminokislotani kodlovchi 1444 nukleotid mavjud. 17 ta muqobil ravishda qo'shilgan 1 ta nusxa ko'chirilmagan varianti mavjud.[11]

Gomologiya

Paraloglar

Bittasi bor paralog TMEM176B bo'lgan TMEM176B.[12]

Ortologlar

Bu ortologlar jadvaliga asoslangan ildiz otmagan filogenetik daraxtdir. Qora guruh baliqlar, qizil guruhlar Yirtqich hayvon, ko'k guruh Artiodaktila va Sireniya, yashil guruh Rodentiya, va to'q sariq guruh Primatlar

Quyida jadval mavjud ortologlar TMEM176B-ga yaqin va biroz uzoq orloglar kiradi.

Umumiy ismKirish raqamiTartib uzunligiKetma-ket identifikatsiyaKetma-ket o'xshashlik
InsonNP_054739.3270100100
BonoboXP_008966185.12869998
Uganda Qizil KolobusXP_023081229.12689192
Kichik quloq GalagoXP_003792108.12686877
Sunda Flying LemurXP_008564209.12717180
Uy sichqonchasiNP_001273581.12635366
Ryukyu sichqoniXP_021020106.12655367
Prjevalskiy otiXP_008506799.12707080
GepardXP_026907001.12716877
BuffaloXP_006049155.12656676
Yovvoyi YakXP_005908946.12656575
Sperma kitXP_028345261.12666573
Qotil kitXP_012392713.12656473
Tinch okean morsiXP_004408899.12716475
Gigant pandaXP_019651134.12716879
Qizil tulkiXP_025849593.12736373
G'arbiy Hind ManateiXP_004372786.12716676
KoalaXP_020833594.12684662
Osiyo ArowanaXP_018595194.12523847
Shimoliy PikeXP_010904195.42512646

Genlarning primatlaridan sichqonlarga va baliqlarning ayrim turlariga qadar 125 ga yaqin ortologlari mavjud.[13]

Gomologlar

Ushbu genning gomologlariga shimpanze, Rhesus maymuni, it, sigir, sichqon va kalamush.[14]

Oqsil

TMEM176B ikkilamchi tuzilish modeli. Ushbu tuzilish membrana oqsilidir va voltajga bog'liq bo'lgan gamma-8 kaltsiy kanalidir.

TMEM176B ning molekulyar og'irligi 29,1 kiloDalton (kdal) dir. Protein juda boy valin va kambag'al aspartik kislota.[15] TMEM176B isoform a tarkibida 4 ta transmembran mintaqalar mavjud.[16] 198-687 gacha bo'lgan CD20 domeni mavjud.[17]

Domenlar

CD20 ga o'xshash oila CD20 genini o'z ichiga oladi, ammo cl04401 superfamilyasiga kiruvchi pfam04103 oilasining bir qismidir. Ushbu aniq domen mintaqasi 489 bp.[18]

Ikkilamchi tuzilish

TMEM176B alfa-spirallardan, beta-simlardan va TM-spirallardan iborat. Alfa spirallari ikkilamchi strukturaning ko'p qismini, so'ngra TM spirallarini tashkil qiladi.[19]

Subcellular localization

Asosan Golji apparatida lokalize qilingan, ammo qo'shimcha ravishda plazma membranasi va nukleoplazmasida joylashgan.[20]

Protein-oqsilning o'zaro ta'siri

Protein ko'pincha TMEM176A bilan o'zaro ta'sir qiladi. Shuningdek, u o'zaro ta'sir qiladi TMEM47 va CPXM1 (karboksipeptidaza 1), ammo past darajalarda.[21]

TMEM176B o'zaro ta'sir qiluvchi oqsillarning diagrammasi.

Tartibga solish

Gen

TMEM176B-da 11 ta promouter mavjud. A izoform a oldidan promotor mintaqa 1101 bp bo'lib, 150,799,077-150,800,177 ni o'z ichiga oladi.[22]

Oqsil

TMEM176B izoform a-da 4 ta fosforillanish joylari mavjud.[23]

Klinik ahamiyati

TMEM176B geni g'ayritabiiy ravishda to'planganda saraton kasalligi bilan bog'liqligini ko'rsatadigan tadqiqotlar mavjud.[24]

Adabiyotlar

  1. ^ a b v GRCh38: Ensembl relizi 89: ENSG00000106565 - Ansambl, 2017 yil may
  2. ^ a b v GRCm38: Ensembl relizi 89: ENSMUSG00000029810 - Ansambl, 2017 yil may
  3. ^ "Human PubMed ma'lumotnomasi:". Milliy Biotexnologiya Axborot Markazi, AQSh Milliy Tibbiyot Kutubxonasi.
  4. ^ "Sichqoncha PubMed ma'lumotnomasi:". Milliy Biotexnologiya Axborot Markazi, AQSh Milliy Tibbiyot Kutubxonasi.
  5. ^ Picotto G, Morse L, Nguyen N, Saltzman J, Battaglino R (iyul, 2019). "TMEM176A va TMEM176B umurtqaning surunkali shikastlanishidan so'ng dendritik hujayraning kamolotini va funktsiyasini inhibe qilishning nomzod regulyatorlari". Neurotrauma jurnali. 37 (3): 528–533. doi:10.1089 / neu.2019.6498. PMC  6978786. PMID  31354034.
  6. ^ a b Umumiy oqsil resurslariga qo'shilish raqami Q3YBM2 "Transmembran oqsili 176B" uchun UniProt.
  7. ^ "TMEM176B Transmembran oqsili 176B [Homo Sapiens (Inson)] - Gen - NCBI". Ncbi.Nlm.Nih.Gov, 2019 yil, https://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=full_report&list_uids=28959.
  8. ^ "Homo Sapiens xromosomasi 7, Grch38.P13 Birlamchi yig'ilish - Nukleotid - NCBI". Ncbi.Nlm.Nih.Gov, 2019 yil, https://www.ncbi.nlm.nih.gov/nuccore/NC_000007.14?report=fasta&from=150791287&to=150801360&strand=true.
  9. ^ "Geneloc 7-xromosoma uchun xaritasi: qidiruv natijalari". Genecards.Weizmann.Ac.Il, 2019, https://genecards.weizmann.ac.il/geneloc-bin/display_map.pl?chr_nr=7&range_type=gc_id&gc_id=GC07M150791#GC07M150791.
  10. ^ Genecards.Org, 2019, https://www.genecards.org/cgi-bin/carddisp.pl?gene=LOC105375566.
  11. ^ Danielle Thierry-Mieg va Jean Thierry-Mieg, [email protected]. "Aceview: Geneid: TMEM176B, Mrnas yoki Estsaceview bilan inson, sichqon va qurtlarni genlariga keng tushuntirish.". Ncbi.Nlm.Nih.Gov, 2019 yil, https://www.ncbi.nlm.nih.gov/IEB/Research/Acembly/av.cgi?c=geneid&org=9606&l=28959.
  12. ^ "Gen: TMEM176A (ENSG00000002933) - Paraloglar - Homo Sapiens - Ensembl Genome Browser 97". Useast.Ensembl.Org, 2019, http://useast.ensembl.org/Homo_sapiens/Gene/Compara_Paralog?g=ENSG00000002933;r=7:150800403-150805118.
  13. ^ "TMEM176B Ortologlari". NCBI, 2019, https://www.ncbi.nlm.nih.gov/gene/28959/ortholog/?scope=117570.
  14. ^ Gomologen - NCBI ". Ncbi.Nlm.Nih.Gov, 2019, https://www.ncbi.nlm.nih.gov/homologene/8521.
  15. ^ "SAPS natijalari". Ebi.Ac.Uk, 2019, https://www.ebi.ac.uk/Tools/services/web/toolresult.ebi?jobId=saps-I20190730-112615-0877-10414538-p2m.
  16. ^ 2019, https://psort.hgc.jp/cgi-bin/runpsort.pl. Kirish 4 Avgust 2019.
  17. ^ "Transmembran oqsili 176B Isoform A [Homo Sapiens] - Protein - NCBI". Ncbi.Nlm.Nih.Gov, 2019 yil, https://www.ncbi.nlm.nih.gov/protein/NP_054739.3.
  18. ^ "CDD konservalangan proteinli domen oilasi: CD20". Ncbi.Nlm.Nih.Gov, 2019 yil, https://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=309291.
  19. ^ "Phyre 2 natijalari uchun aniqlanmagan". Sbg.Bio.Ic.Ac.Uk, 2019 yil, http://www.sbg.bio.ic.ac.uk/phyre2/phyre2_output/607daef47ac621bf/summary.html.
  20. ^ "Hujayra atlasi - TMEM176B - Inson oqsil atlasi". Proteinatlas.Org, 2019, http://www.proteinatlas.org/ENSG00000106565-TMEM176B/cell.
  21. ^ "TMEM176B oqsillari (inson) - STRING o'zaro aloqalar tarmog'i". String-Db.Org, 2019, https://string-db.org/cgi/network.pl?taskId=YtRoRnnnUnu6.
  22. ^ "Genomatix". Genomatix.De, 2019, https://www.genomatix.de/cgi-bin/eldorado/eldorado.pl?s=31f15c28bad5fbc98bd01817d3f6f82f;SHOW_ANNOTATION=result_1;ELDORADO_VERSION=E34R1811.
  23. ^ Transmembran oqsili 176B Isoform A [Homo Sapiens] - Protein - NCBI ". Ncbi.Nlm.Nih.Gov, 2019, https://www.ncbi.nlm.nih.gov/protein/NP_054739.3.
  24. ^ Cuajungco MP, Podevin V, Valluri VK, Bui Q, Nguyen VH, Teylor K (Noyabr 2012). "Odam transmembranasi (TMEM) -176A va 176B oqsillarining g'ayritabiiy to'planishi saraton patologiyasi bilan bog'liq". Acta Histochemica. 114 (7): 705–12. doi:10.1016 / j.acthis.2011.12.006. PMC  5419829. PMID  22244448.